--- title: An enzyme-free, cold-process acoustic method for tissue dissociation description: The differences observed in cell surface expression suggest that cells dissociated using enzymes and heat experience stress-induced changes post-harvest. image: https://blog.cellsonics.com/hubfs/Cell-Membrane-Blog-Tile_web.jpg --- [Skip to main content](https://blog.cellsonics.com/blog/biorxiv#main) [![Cellsonics](https://blog.cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/cellsonics-logo.svg) ![Cellsonics-logo](https://blog.cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/Cellsonics-logo.svg)](https://cellsonics.com/home-new?hs_preview=ggrFiNrO-179903288415) - [Resources](https://cellsonics.com/resources) - [About Us](https://cellsonics.com/about-us) - [Careers](https://cellsonics.com/careers) - [Blog](https://blog.cellsonics.com/blog) - [Contact Us](https://cellsonics.com/contact-us) Open main navigation Close main navigation - [Resources](https://cellsonics.com/resources) - [About Us](https://cellsonics.com/about-us) - [Careers](https://cellsonics.com/careers) - [Blog](https://blog.cellsonics.com/blog) - [Contact Us](https://cellsonics.com/contact-us) October 9, 2023 # An enzyme-free, cold-process acoustic method for tissue dissociation ![Melanie MacMullan, PhD](https://blog.cellsonics.com/hs-fs/hubfs/Melanie.jpg?width=50&height=50&name=Melanie.jpg) by [Melanie MacMullan, PhD](https://blog.cellsonics.com/blog/author/melanie-macmullan-phd) ## Read the preprint of the Cellsonics Manuscript at BioRxiv ## Abstract As biological advances continue to improve the resolution of genomic and proteomic studies, the quality of single cell suspensions is becoming increasingly important. While conventional approaches use enzymes which may require heat Abbreviations: Bulk Lateral Ultrasound (BLU) activation to break down extracellular tissue matrices and gain access to single cells, recent studies have suggested that these harsh biochemical and heat-based treatments may result in genomic and proteomic modulation. To minimize these dissociation artifacts, we have developed an instrument for dissociating cells from various tissue matrices using Bulk Lateral Ultrasound (BLU™) energy. This enzyme-free, gentle mechanical dissociation maintains sample temperatures below 8°C for the duration of processing, resulting in high-fidelity single cell suspensions with comparable viability and live cell counts to those obtained with conventional enzymatic dissociations. Here, in murine-derived brain, heart, lung, and B16 melanoma tumor tissue dissociated by either BLU or by a commercially available dissociation kit which uses enzymes and heat, we compare cell viability and expression of population-specific immunological markers. The dramatic differences observed in cell surface expression suggest that cells dissociated using enzymes and heat may be experiencing stress-induced changes post-harvest that could impact conclusions and impede research progress. Alternatively, using gentle mechanical dissociation with BLU, we demonstrate the preservation of these markers and enable a minimally invasive alternative to obtaining high integrity single cell suspensions. [Download here](https://www.biorxiv.org/content/10.1101/2023.10.03.560704v1.full.pdf) ### Interested in learning more? Ask for a 20-minute seminar from a Cellsonics Scientist [Get Seminar](https://cellsonics.com/get-seminar) ## Related Posts ##### [![Enzyme-free dissociation preserves critical mouse lung cell markers](https://blog.cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Blog/Enhanced-Mouse.jpg?width=520&height=294&name=Enhanced-Mouse.jpg) September 1, 2023 Enzyme-free dissociation preserves critical mouse lung cell markers](https://blog.cellsonics.com/blog/mouse-lung) ##### [![Enhanced Mouse Brain Cell Surface Expression With SimpleFlow](https://blog.cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Blog/Enzyme-free.jpg?width=520&height=294&name=Enzyme-free.jpg) August 1, 2023 Enhanced Mouse Brain Cell Surface Expression With SimpleFlow](https://blog.cellsonics.com/blog/mousebrain) ##### [![Rapid Skin Dissociation Protocol using acoustic energy](https://blog.cellsonics.com/hs-fs/hubfs/Cellsonics%20-%202024/Images/Blog/Skin.jpg?width=520&height=294&name=Skin.jpg) October 18, 2023 Rapid Skin Dissociation Protocol using acoustic energy](https://blog.cellsonics.com/blog/mouse-skin) #### Subscribe to be notified about the latest posts. #### Sign up to be sent application notes. [Sign Up](https://cellsonics.com/sign-up) [![CellsonicsLogoWhite(2048 x 366 px)](https://blog.cellsonics.com/hs-fs/hubfs/CellsonicsLogoWhite(2048%20x%20366%20px).png?width=300&height=54&name=CellsonicsLogoWhite(2048%20x%20366%20px).png "CellsonicsLogoWhite(2048 x 366 px)")](https://cellsonics.com/home-new?hs_preview=ggrFiNrO-179903288415) #### Location 76 Bonaventura Drive San Jose, CA 95134 #### Contact - [Linkedin](https://www.linkedin.com/company/cellsonics/) - [Email](mailto:Cellsonics-Communications@Cellsonics.com) - [Privacy Policy](https://cellsonics.com/privacy-policy) - [Terms of Service](https://cellsonics.com/terms-and-conditions) Copyright © 2026, Cellsonics. All Rights Reserved ![](https://px.ads.linkedin.com/collect/?pid=5165674&fmt=gif) ```json { "@context" : "https://schema.org", "@type" : "BlogPosting", "author" : { "@type" : "Person", "name" : "Melanie MacMullan, PhD", "url" : "https://blog.cellsonics.com/blog/author/melanie-macmullan-phd" }, "dateModified" : "2025-02-10T18:33:08.689Z", "datePublished" : "2023-10-09T16:08:51.000Z", "headline" : "An enzyme-free, cold-process acoustic method for tissue dissociation", "image" : [ "https://blog.cellsonics.com/hubfs/Cell-Membrane-Blog-Tile_web.jpg" ], "mainEntityOfPage" : { "@id" : "https://blog.cellsonics.com/blog/biorxiv", "@type" : "WebPage" }, "publisher" : { "@type" : "Organization", "logo" : { "@type" : "ImageObject", "url" : "https://blog.cellsonics.com/hubfs/Cellsonics%20-%202024/Images/Global/cellsonics-logo.svg" }, "name" : "Cellsonics" } } ```